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A mean graph analysis depicts the relative sensitivity of the various cell lines, grouped according to disease category, as shown in Figure 1. This format allows the identification of compounds or extracts that display inhibitory activity for specific categories of disease, as shown in Figure 1. More complex computer-based analyses of the screening results include the following : 1. A COMPARE program that searches for compounds displaying a pattern of activity matching the profile of a lead compound [10].

J Clin Oncol 6 : 1653-1664 Van Kalken CK, Pinedo HM, Giaccone G (1991) Multidrug resistance from the clinical point of view. Eur J Cancer 27 : 1481-1486 Miller TP, Grogan TM, Dalton WS et a! (1991) P-Glycoprotein expression in malignant lymphoma and reversal of clinical drug resistance with chemotherapy plus highdose Verapamil. J Clin Oncol 9 : 17-24 Giaccone G, Gazdar AF, Beck H et a! (1992) Multidrug sensitivity phenotype of human lung cancer cells associated with topoisomerase II expression.

G. Verapamil). P-gp is expressed in several normal human tissues and although a detoxifying function, a role in Cortisol, Aldosterone and Bilirubin transport and a role in regulating epithelial cell volume have been proposed. Increased P-gp expression after chemotherapy has been reported in neuroblastoma, sarcoma, breast cancer, ovarian cancer, nephroblastoma and several hematological malignancies, while an association between P-gp expression and worse prognosis has been described in neuroblastoma, sarcoma and some hematological malignancies.

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3-D Designs


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