Download PDF by Wayne W Umbreit: Advances in Applied Microbiology, Vol. 3

By Wayne W Umbreit

ISBN-10: 0120026031

ISBN-13: 9780120026036

Meant for researchers in utilized microbiology and environmental engineers, this publication covers such subject matters as environmental evaluation of biotechnological approaches and microbial ameliorations of haloaromatic and haloaliphatic compounds.

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Naylor and Smith (1946) found that S. mrcescens grown at 30° to 34OC. for 18 to 24 hours was more resistant to drying than cultures grown for longer or shorter periods. Benedict et al. (1958) not only confirmed the general observations of Naylor and Smith, but showed that, with constant aeration, cultures incubated at 29O or 3OOC. were significantly more resistant to lyophilization than those cultured at 21° or 25OC. although the total viable cell counts were comparable. , the highest aeration rate yielded the most resistant cultures.

Survival was higher if the cultures were harvested after the pigment was fully developed. It was suggested that the pigment had nothing to do with survival but that pigmentation was merely a property of older cultures and that young cultures were sensitive to lyophilization. 28 ROBERT J. , the culture is at the maximum stationary phase. C. CELLCONCENTRATION The highest concentration of cells which can be conveniently prepared and dispensed is ideal since the objective is to obtain dry cells and any unnecessary water is avoided.

Others believe, however, that it is possible to dry organisms excessively and that one should allow a certain amount of moisture to remain. Stamp ( 1947), Fry and Greaves (1951), and others have added glucose (which is hygroscopic) to culture suspensions immediately before drying to assure some residual moisture. Muggleton (1960) contended that com- 44 ROBERT J. HECKLY plete removal of water is incompatible with life, at least with respect to the tubercle bacillus. He also added glucose to retain moisture but he did not show that the beneficial effect was due to increased moisture rather than to the presence of glucose per se.

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Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit

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