Analytical Chemistry of Bacillus thuringiensis by Leslie A. Hickle, William L. Fitch PDF

By Leslie A. Hickle, William L. Fitch

ISBN-10: 0841212880

ISBN-13: 9780841212886

ISBN-10: 0841218153

ISBN-13: 9780841218154

content material: Analytical chemistry of Bacillus thuringiensis : an outline / Leslie A. Hickle and William L. Fitch --
ancient facets of the quantification of the energetic component percent for Bacillus thuringiensis items / George Tompkins, Reto Engler, Michael Mendelsohn, and Phillip Hutton --
Bioassay tools for quantification of Bacillus thuringiensis [delta]-endotoxin / Clayton C. Beegle --
Specificity of insecticidal crystal proteins : implications for business standardization / R. Milne, A.Z. Ge, D. Rivers, and D.H. Dean --
In vitro analyses of Bacillus thuringiensis [delta]-endotoxin motion / George E. Schwab and Paul Culver --
id of entomocidal pollution of Bacillus thuringiensis through high-performance liquid chromatography / Takashi Yamamoto --
Characterization of parasporal crystal pollution of Bacillus thuringiensis Subspecies kurstaki lines HD-1 and NRD-12 : use of oligonucleotide probes and cyanogen bromide mapping / L. Masson, M. Bossé, G. Préfontaine, L. Péloquin, P.C.K. Lau, and R. Brousseau --
improvement of a high-performance liquid chromatography assay for Bacillus thuringiensis var. san diego [delta]-endotoxin / Leonard Wittwer, Denise Colburn, Leslie A. Hickle, and T.G. Sambandan --
Use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to quantify Bacillus thuringiensis [delta]-endotoxins / Susan M. Brussock and Thomas C. Currier --
Quantitative immunoassay of insecticidal proteins in Bacillus thuringiensis items / R. Gene Groat, James W. Mattison, and Eric J. French --
The light-scattering characterization of [delta]-endotoxin creation in inclusion our bodies / Fritz S. Allen, Betty J.M. Hannoun, Tammy B. Hebner, and Kathryn Nette --
Quantification of Bacillus thuringiensis insect regulate protein as expressed in transgenic crops / Roy L. Fuchs, Susan C. MacIntosh, Duff A. Dean, John T. Greenplate, Frederick J. Perlak, Jay C. Pershing, Pamela G. Marrone, and David A. Fischhoff --
High-performance liquid chromatography research of 2 [beta]-exotoxins produced by way of a few Bacillus thuringiensis lines / Barry L. Levinson.

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Extra resources for Analytical Chemistry of Bacillus thuringiensis

Sample text

Because of the lack of knowledge about the structure of B. thuringiensis insecticidal crystal proteins, past discussions about the specificities of crystal proteins tend to emphasize host processes which act upon crystal proteins rather than intrinsic properties of proteins themselves. Aside from host factors such as insect midgut proteases, pH, and types of crystal protein toxin receptors, the specificities of insecticidal crystal proteins seem to be determined by amino acid sequences located on the variable domains of these proteins.

Virescens. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. 4. MILNE ET AL Specificity of Insecticidal Crystal Proteins 33 An apparent exception to this is the recent results of Wolfersberger (54) on L. dispar which indicates an inverse relationship between binding affinity (Kd) and specificity. 03 nM respectively). Implications for Industrial Standardization The recognition that insecticidal crystal proteins are primary agents in insecticidal activity of B. thuringiensis toward certain insects opens the way for the development of simplified assay conditions.

In the case of A. kuehniella, susceptibility was demonstrated toward B. thuringiensh Berliner crystal proteins when applied with spores, or if dissolved and activated prior to ingestion (30). H. virescens is very susceptible to B. 3) if the crystal is solubilized prior to ingestion (25). Similar observations have been noted for the European corn borer (31), however, solubilization does not increase activity of some crystal proteins. Crystal protein of B. thuringiensis aizawai (HD-249) did not display activity toward O.

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Analytical Chemistry of Bacillus thuringiensis by Leslie A. Hickle, William L. Fitch


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