Download e-book for kindle: Biotechnology, Bioprocessing by G. Stephanopoulos

By G. Stephanopoulos

ISBN-10: 1560811536

ISBN-13: 9781560811534

ISBN-10: 1560816023

ISBN-13: 9781560816027

ISBN-10: 3527283102

ISBN-13: 9783527283101

ISBN-10: 3527283137

ISBN-13: 9783527283132

Bioprocessing: an exhilarating new engineering self-discipline. It combines the improvement and optimization of biotechnological techniques with powerful concepts to get well and purify the specified items. protection in addition to fee play a tremendous function right here. This quantity covers the immensely differentiated spectrum of ideas and operations of bioprocessing, awarded by way of the main useful specialists within the box. an outline of upstream and downstream processing is given, fermentation and mobile tradition techniques and the layout of microbial fermenters are offered. A final crew of chapters is devoted to problems with strategy validation, size, and rules. subject matters integrated are: commercial mobilephone Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen move/ Scale Implications/ Fermentation info research/ mobilephone and particles elimination/ Protein Purification/ Electrokinetic Separations/ ultimate restoration Steps/ technique Validation

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5 Influence of Product Recovery of metabolite concentration by analytical techniques such as HPLC and GLC. A novel approach has been the use of near infrared (NIR) spectroscopy, which may be adapted for online measurement using a fiber optic module (HAMMOND and BROOKES,1992). The authors describe the use of NIR for determination of antibiotics (Fig. 7). portant to understand the impact of the fermentation conditions on the subsequent processing operations (FISH and LILY, 1984). Major improvements can be made by correct selection of the organism, culture medium, and growth conditions.

Future research must address these gas-free systems occurs at much higher agita- aspects of gradients, and reduce them in order tion, as the Kolmogorov eddy length decreases to eliminate questions of product consistency. to that of the single cell. 4 Application of Biosensors moderate agitation rates (PAPOUTSAKIS, 1991).

Cell damage is rarely duction of human therapeutics. , however (Fox, 1992). Homogegion off the impeller or a close clearance be- neous systems such as stirred tank bioreactors tween the impeller and the vessel wall. In mi- ensure that each cell experiences the same envicrocarrier cultures, two neighboring microcar- ronment and culture conditions. Heterogeneriers might collide with each other due to the ous bioreactors, however, may have culture action of turbulent eddies. By understanding conditions which vary within the bioreactor.

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Biotechnology, Bioprocessing by G. Stephanopoulos

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