By Dean F. Martin and Barbara B. Martin
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Extra resources for Column Chromatography
Figure 13. Separation of methylamines and methylamine-N-oxides from standard solutions. Analytes: 1-sodium, 2ammonium, 3-methylamine (195 µg/L), 4-dimethylamine (390 µg/L), 5-trimethylamine-N-oxide (465 µg/L), and 6-tri‐ methylamine (615 µg/L) . 5772/55652 Figure 14. Chromatogram of smog chamber filter analysis from reaction of trimethylamine with ozone. 57 µg/m3). The inset is a magnification of the trimethylamine-N-oxide peak (5) from the chromatogram . 3. Phenolic compounds Phenolic compounds have attracted great concern in recent years due to their high toxicity and bio-recalcitrant effect in the ecosystem water cycling process.
Silva P. , Malloy Q. G. , Cocker D. R. & Purvis-Roberts K. L. Determination of methylamines and trimethylamine-N- oxide in particulate matter by non-suppressed ion chromatography. Journal of Chromatogra‐ phy A; 2010; 1217(13) 2070-2073.  Moldoveanu S. C. & Kiser M. Gas chromatography/mass spectrometry versus liquid chromatography/fluorescence detection in the analysis of phenols in mainstream cig‐ arette smoke Journal of Chromatography A 2007; 1141(1) 90-97. , Stokke T. , Jgensen, K. B. & Beyer J.
In order to prevent variation in matrix and protein net charge, maintenance of a constant mobile phase pH during separation is essential to avoid pH changing which can occur when both protein and exchanger ions are released into the mobile phase . By means of buffer substances pH value can be controlled and adjusted. Concentration of H+ and the buffering component influence the protein binding to the stationary phase, chromatographic resolution and structural as well as functional integrity of the protein to be separated.
Column Chromatography by Dean F. Martin and Barbara B. Martin