New PDF release: Gas Enzymology: Proceedings of a Symposium held at Odense

By Sándor Bohátka (auth.), H. Degn, R. P. Cox, H. Toftlund (eds.)

ISBN-10: 9400952791

ISBN-13: 9789400952799

ISBN-10: 9401088314

ISBN-13: 9789401088312

Being small, shapeless and inert a gasoline molecule doesn't appear to be an enzyme's dream of a substrate. however evolution has supplied a bunch of enzymes that may engage particularly with gasoline molecules resembling oxygen, carbon dioxide, nitrogen, hydrogen and so on. a lot of those enzymes play dominant roles at the global scene in biogeochemical cycles. at the mobile point they generally tend to be heavily attached to the power maintaining gear. We outline gasoline Enzymology because the research of those enzymes. traditionally, gasoline Enzymology is a subspecialty of bioenergetics. Its foundations, technical in addition to conceptual have been laid through Warburg in his reports of the mobile combustion of foodstuff. The Warburg gear supported the 1st thirty years of analysis within the box. It was once succeeded via the Clark electrode which had its heyday through the interval while the trendy suggestions of bioenergetics took form. The Clark electrode, itself imminent thirty years of age, is now being sup­ plemented and every so often changed by way of the tremendously extra robust membrane inlet mass spectrometer which measures with equivalent ease all dis­ solved gases of curiosity in biochemistry. it's our trust that destiny improvement of fuel Enzymology should be associated with the frequent make the most­ ation of this technique.

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Additional info for Gas Enzymology: Proceedings of a Symposium held at Odense University, Denmark, 28–29 May 1984

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_·:"- r"")/ 1/ Ii -. r· ·· ...... ••• 40. ,-- .... ••• ! length Inm) Figure 5. ) of methanolgrown Candida boidinii. Difference spectra anaerobic (+300 mH methanol) - aerobic (>10 pli 02) were obtained at times: spectrum 1, 0 min; spectrum 2, 20 min; spectrum 3, 73 min; spectrum 4, 540 min. 80 ---.. '" :> !.. :. tI " • eo:• 30 ~ 51 . •" 20. 4 •"" <; • 10 : • • C -.. tI 00 2 3 Tim. (hi • 5 -- .. 60 • iL . 20 :: OJ 0 Figure 6. "Catabolite inactivation" by ethanol (174 mIl) of methanolgrown C.

FEUS Microbiol. Lett. 11, 143-146'. , UELLOR, H. L. (1983). 47-51. (22) VAUGHAN, W. & WEBER, G. (1970). Biochem. ~, Biochem. J. , 464-473. G. S. (1980). Biophys. Acta. 591, 187-197. Biochim. A. H. (1981). J. Cell Physiol. 107, 329-334. (25) PASTEUR, L. (1861). R. Acad. , Paris. A. (1972). , 1-27. , KRISTENSEN, B. DEGN, H. (1982). 126, 167-170. P. & LLOYD, D. (1984). (29) JONES, S. & LLOYD, D. (1984). (30) HOCH, G. &- KOK, B. 160-170. (1963). 52, 1260-1265. J. Gen. Microbiol. Unpublished data. Unpublished data.

Unpublished data. Unpublished data. Arch. Biochem. Biophys. , COMBINED SPECTROPHOTOHETRIC AND OXYGEN HEASUREIIENTS DURING ETHANOL INACTIVATION D. Hill and D. K. ABSTRACT. The respiratory metabolism of the methanol-srown yeast C. boidinii was studied using combined spectrophotometric and oxygen measurements, with particular reference to the peroxisomal enzyme FADlinked alcohol oxidase. 6 pM. 99 pM (4). Oxygenation in the presence of methanol (300 mM) results in oxidation of only about 30% of the total FAD oxidised in the absence of substrate.

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Gas Enzymology: Proceedings of a Symposium held at Odense University, Denmark, 28–29 May 1984 by Sándor Bohátka (auth.), H. Degn, R. P. Cox, H. Toftlund (eds.)

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