By Raymond Turpin, Jérôme Lejeune, P. Alexander and Z. M. Bacq (Auth.)
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F) Filming The quality of the staining is a primary condition for the quality of the negatives and that is why we prefer staining with Unna's blue which gives excellent contrast. When the contrast is not distinct enough phase contrast may be useful. However, a certain loss in definition of the image during phase contrast and the insufficient planeity of modern optics limits its use when the preparations are properly stained. Its only merit is that it enables one to salvage imperfect material. However, it should be pointed out, by way of curiosity, that it is perfectly possible to photograph unstained preparations with phase contrast.
This simple method gives very faithful results and it is quite remarkable to note the highly concordant results in the hands of very different workers using different techniques on tissues of the most diverse origin. The second stage is to describe by one number the length of each chromosome and by a second, the position of its centromere. The accumulation of many karyotypes can then be used to calculate a mean value for these two parameters and to say, for example, chromosome 1 has such a length and its centromere is found in such a position.
J. " " '1 \ ' 25 • I " .... , 30 . '. : .. lt Length . ' \, . '\ . :. \. I ••\' . ', ..... , .. ';. . ,'. : :t-': . ,. , . -'.. ' II . - ~ :: . ~ . '\"~I ,. ,' ...... J'\ I. ~. , ',,, '0' • . ~ . ,,'I. 12 -,i',) . _ ; . '. ~ <~ j ;: '<, "- 35 I E 25 a~ 30 c . -~ . / 30 c9 C~ 35 S N O I I V H ^ a a V IVIMOSOJMOtfHO QMV S N O I 1 3 I T J J V MVWflH NORMAL HUMAN KARYOTYPE 51 (1962d) in a systematic study observed that the mean number of satellites per cell was higher for Feulgen than for orcein preparations.
Human Afflictions and Chromosomal Aberrations by Raymond Turpin, Jérôme Lejeune, P. Alexander and Z. M. Bacq (Auth.)