By Kennedy Omondi Okeyo, Hiromi Miyoshi, Taiji Adachi
This e-book covers issues on mechanosensing, mechanotransduction, and actin cytoskeletal dynamics in mobilephone motility. it's going to give a contribution to a greater figuring out of ways cells functionally adapt to their mechanical atmosphere in addition to highlighting basic ideas for designing fabric niches for mobilephone manipulation. With subject matters from multidisciplinary fields of the lifestyles sciences, medication and engineering, the ebook is the 1st of its type, delivering finished, built-in insurance of leading edge techniques to mobile biomechanics. It presents a invaluable source for seniors and graduate scholars learning telephone biomechanics and is additionally appropriate for researchers drawn to the appliance of equipment and methods in reference to the cutting edge techniques mentioned. every one portion of the booklet has been supplemented with concrete examples and illustrations to facilitate realizing even for readers strange with telephone biomechanics.
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Additional info for Innovative Approaches to Cell Biomechanics: From Cell Migration to On-Chip Manipulation
A close examination of the spatiotemporal dynamics of the actin network flow field can reveal how the F-actin flow changes during the course of cell movement relative to such parameters as cell shape. This is important because cell shape is a balance of polymerization-mediated leading edge protrusion that result in cell-shape expansion, and retraction of the cell rear which is mediated by actomyosin contractility and results in cell-shape contraction. In fish keratocytes, this balance is well coordinated with protrusion and retraction occurring at a uniform pace resulting in the maintenance of crescent-moon shape.
This is important because cell shape is a balance of polymerization-mediated leading edge protrusion that result in cell-shape expansion, and retraction of the cell rear which is mediated by actomyosin contractility and results in cell-shape contraction. In fish keratocytes, this balance is well coordinated with protrusion and retraction occurring at a uniform pace resulting in the maintenance of crescent-moon shape. Spatiotemporal dynamics of actin network flow in the course of a fragment’s movement is shown in the flow intensity color maps of Fig.
1 Cellular Fragments as Simplified Motility Systems Fragment Formation from Fish Keratocytes Fragments can be formed from fish keratocytes cultured for 24 h in DMEM (Dulbecco’s Modified Eagle’s Medium) supplemented with 15 % FBS. For more details, refer to the procedures described in (Adachi et al. 2009). Typically, fragmentation can be induced in migrating cells such a fish keratocytes by treating them with drugs that compromise the stability of the actin-myosin system responsible for maintaining an intact cellshape.
Innovative Approaches to Cell Biomechanics: From Cell Migration to On-Chip Manipulation by Kennedy Omondi Okeyo, Hiromi Miyoshi, Taiji Adachi