Download PDF by N.A. Parris: Instrumental Liquid Chromatography

By N.A. Parris

ISBN-10: 0080858058

ISBN-13: 9780080858050

ISBN-10: 0444414274

ISBN-13: 9780444414274

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The recorded peak, however, indicates the total dispersion of a sample during its passage through the apparatus. It is important t o distinguish between dispersion of the peak which takes place within the column, due largely to the nature of the column packing material, and dispersion or mixing which can occur before or after the column, in places such as the injector, the interconnecting tubing, and the detector. This extra-column band broadening becomes progressively more important as high efficiency is demanded from the equipment and when high-performance columns are used it can become the limiting feature if insufficient attention has been paid to the design of these parts.

When lengths of columns greater than these are required, it is common practice to couple two or more columns in series, using lowvolume capillary connectors. Various designs have been proposed for column connectors. D. 32 THE CHROMATOGRAPHIC SUPPORT AND COLUMN Fig. 5. Construction o f a low-dead-volume coupling for connecting two columns. Nuts, ferrules and columns have been omitted for clarity sake. ). capillary. 1). Columns having internal diameters in the range 1-5 mm are used for analytical separations, whereas the larger sizes tend to be used for either steric exclusion chromatography or preparative separations.

Construction o f a low-dead-volume coupling for connecting two columns. Nuts, ferrules and columns have been omitted for clarity sake. ). capillary. 1). Columns having internal diameters in the range 1-5 mm are used for analytical separations, whereas the larger sizes tend to be used for either steric exclusion chromatography or preparative separations. The development of packing techniques for supports of very small diameter (5-10 pm) has resulted in columns of such high efficiency that short lengths, i e .

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Instrumental Liquid Chromatography by N.A. Parris


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