By Prof. Dr. Jean Brachet, Dr. Henri Alexandre (auth.)
Nearly 10 years have elapsed in view that i ended writing the 1st variation of Intro duction to Molecular Embryology. in this interval, molecular embryology has made nice strides ahead, yet with out present process a tremendous revolution; there fore, the overall philosophy and description of the publication have remained virtually un replaced. even if, the entire chapters needed to be nearly thoroughly rewritten in or der to introduce new evidence and to do away with findings that have misplaced curiosity or were disproved. there has been an enormous hole within the first version of this publication: little or no was once acknowledged approximately mammalian eggs regardless of their seen curiosity for mankind. learn on mammalian eggs and embryos is so energetic at the present time that this crucial subject merits a whole bankruptcy in a publication curious about molecular embryology. as a result, i'm very grateful to my colleague Dr. Henri Alexandre, who has written a bankruptcy on mammalian embryology (Chap. nine) and has ready the entire illustrations for this book.
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Extra info for Introduction to Molecular Embryology
Driesch). a Normal development leading to the formation of a pluteus larva. b The separation of the two first blastomeres leads to the formation of two smaller, but normal plutei into a UV-irradiated egg allows the formation of normal ovaries or testes. The nature of the active material is not known, but its strong UV-sensitivity indicates that nucleic acid is an essential factor. In other insects (Cecidyomidae), the nuclei which, during cleavage, reach the germ plasm, retain their chromosomes in the usual way during mitosis; in the other parts of the same eggs, a loss of chromosomes is observed.
14 a-d. Gastrulation in an amphibian embryo. a Early gastrula. Left: dorsal view; right: lateral view. b Middle gastrula. Left: dorsal view; right: section. c Late gastrula at the yolk plug stage. Left: dorsal view; right: vertical section. d Same stage as c transversal section . 2 A Few Important Experimental Facts It is customary, but not quite correct, to divide eggs into two different categories, on the basis of their reactions to experimental approaches such as destroying a part of the egg by pricking, transplanting or explanting groups of cells, centrifuging the egg, etc.
E. they are free to accept and bind any kind of RNA which is presented to them. Since these pioneer experiments, Xenopus oocytes have become an ideal system for testing whether an RNA isolated from any kind of tissue is a messenger. If so, synthesis of the encoded protein takes place in injected oocytes. Recent work has shown that the oocyte is even capable of excreting the foreign proteins if they are normally secreted by secretory cells. In this case, synthesis of the secretory protein, after injection of its mRNA, takes place on ribosomes attached to the oocyte endoplasmic reticulum membrane.
Introduction to Molecular Embryology by Prof. Dr. Jean Brachet, Dr. Henri Alexandre (auth.)