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By John N. Abelson, Melvin I. Simon, Barry L. Karger, William Hancock

ISBN-10: 0121821722

ISBN-13: 9780121821722

The seriously acclaimed laboratory typical for greater than 40 years, tools in Enzymology is likely one of the such a lot hugely revered guides within the box of biochemistry. on account that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. greater than 260 volumes were released (all of them nonetheless in print) and masses of the cloth is appropriate even today--truly a vital booklet for researchers in all fields of lifestyles sciences. Key positive aspects* Liquid chromatography* Electrophoresis* Mass spectrometry

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Lozzi, M. Rustici, A. Santucci, L. Bracci, S. Petreni, P. Soldani, and P. Neri. J. Liq. Chromatogr. 11, 1651 (1988). 77 G. R. Rhodes, M. J. Rubenfield. and C. T. Garvie, J. Chromatogr. 488, 456 (1989). 7s L(F-GC 10, 515 (1992). > D. Guo. C. T. Mant, and R. S. I. (Tzromatogr. 386, 2115 (1987). so C. T. Manl and R. S. Hodges. in "'HPLC of Peptides and Proteins: Separation, Analysis and C o n f o r m a t i o n " (('. T. Mant and R. S. ), p. 327. C R C Press, Boca Raton, F L 1991. s~ H. P. J. B e n n e n , J.

5 in the presence of 5% (Fig. 4, top) or 10% (Fig. 4, bottom) acetonitrile (v/v). Peptides C1-C4 contain, respectively, one to four basic residues (lysine residues) with no acidic residues present. 0 (weak CEC)], the net charges of + 1 to +4 for peptides C1-C4, respectively, do not change. 29 Any variation in retention behavior of the peptide standards would, therefore, be indicative of undesirable pH effects on the cation-exchange matrix. The ~"' W. Kopaciewicz, M. A. Rounds, J. Fausnaugh, and F.

Rustici, A. Santucci, L. Bracci, S. Petreni, P. Soldani, and P. Neri. J. Liq. Chromatogr. 11, 1651 (1988). 77 G. R. Rhodes, M. J. Rubenfield. and C. T. Garvie, J. Chromatogr. 488, 456 (1989). 7s L(F-GC 10, 515 (1992). > D. Guo. C. T. Mant, and R. S. I. (Tzromatogr. 386, 2115 (1987). so C. T. Manl and R. S. Hodges. in "'HPLC of Peptides and Proteins: Separation, Analysis and C o n f o r m a t i o n " (('. T. Mant and R. S. ), p. 327. C R C Press, Boca Raton, F L 1991. s~ H. P. J. B e n n e n , J.

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Methods in High Resolution Separation and Analysis of Biological Macromolecules: Applications, Part B by John N. Abelson, Melvin I. Simon, Barry L. Karger, William Hancock


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