Get Supercritical Fluid Chromatography: Advances and PDF

By Gregory K. Webster

ISBN-10: 9814463000

ISBN-13: 9789814463003

ISBN-10: 9814463019

ISBN-13: 9789814463010

Analytical chemists within the pharmaceutical are constantly searching for more-efficient options to satisfy the analytical demanding situations of today’s pharmaceutical undefined. One method that has made regular advances in pharmaceutical research is supercritical fluid chromatography (SFC). SFC is assembly the chromatography wishes of the by way of delivering effective and selective checking out features at the analytical and preparative scale. The supercritical fluid cellular section, consisting in general of CO2, enables fee relief bills and is helping the in assembly eco-friendly chemistry criteria. This ebook offers a finished evaluate of using SFC in pharmaceutical research.

Supercritical Fluid Chromatography studies using SFC in drug-discovery functions and describes its software in drug improvement. whilst a drug is constructed and taken to industry, it really is established time and again for impurities and degradants, enantiomeric purity, and analytical and preparative isolations—it is confirmed in the course of discovery and improvement and for under-regulated and unregulated methodologies. The publication describes using SFC for every of those purposes and discusses extra in-depth issues, comparable to using SFC in mass spectrometric and polarographic detection. The e-book additionally sheds mild at the position of SFC in drug improvement from traditional items and the development of SFC with new applied sciences and its use in pilot-scale operations as a chromatographic technique.

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Extra resources for Supercritical Fluid Chromatography: Advances and Applications in Pharmaceutical Analysis

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Supercritical fluid chromatography for the 21st century. J Supercrit Fluid, 47(3), 566–573. 15. Ripka, W. , and Krakover, J. (2001). High-throughput purification of compound libraries. Drug Discov Today, 6(9), 471–477. 16. Isbell, J. (2008). Changing requirements of purification as drug discovery programs evolve from hit discovery. J Comb Chem, 10(2), 150–157. 17. , et al. (2004). High-throughput preparative process utilizing three complementary chromatographic purification technologies. J Chromatogr A, 1036(1), 7–13.

Searle, P. A, Glass, K. A, and Hochlowski, J. E. (2004). Comparison of preparative HPLC/MS and preparative SFC techniques for the highthroughput purification of compound libraries. J Comb Chem, 6(2), 175–180. 23. Pinkston, J. , Morand, K. , Tirey, D. , and Stanton, D. T. (2006). Comparison of LC/MS and SFC/MS for screening of a large and diverse library of pharmaceutically relevant compounds. Anal Chem, 78(21), 7467–7472. 24. , and Hsieh, Y. (2008). Supercritical fluid chromatography-mass spectrometry for chemical analysis.

For SFC, 4 min gradient or isocratic methanol in CO2 methods were used with 250 mm columns at a flow rate of 8 mL/min and a system back pressure of 120 bar. A 1 min wash and re-equilibration step was added at the end of each method. By using the Chiralpak AD-H column, the separation between diastereoisomers was possible. 25 26 The Use of SFC in Discovery Sciences The mixture was resolved well into four individual stereoisomers (Fig. 4b) using a Chiralcel OD-H column with an isocratic 4 min method.

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Supercritical Fluid Chromatography: Advances and Applications in Pharmaceutical Analysis by Gregory K. Webster

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