By Bernard Fried
The fourth variation of this paintings emphasizes the overall practices and instrumentation regarding TLC and HPTLC, in addition to their purposes in keeping with compound kinds, whereas supplying an knowing of the underlying idea priceless for optimizing those options. The ebook information up to date qualitative and quantitative densitometric experiments on natural dyes, lipids, antibiotics, prescription drugs, natural acids, pesticides, and extra.
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Extra info for Thin-Layer Chromatography, Revised And Expanded (Chromatographic Science)
1979). Solute-solvent interactions on the surface of silica gel. II. J. Chromatogr. 171: 37-48. Copyright © 1999 by Taylor & Francis Group, LLC Seidler, E. (1995). Experimental estimation of hydrophobic parameters of tetrazolium salts. Acta Histochem. 97: 295-299. , and Guiochon, G. (1981). Optimization in TLC—some practical considerations. J. Chromatogr. 209: 129-147. Stewart, G. H. (1965). The stationary phase in paper chromatography. Adv. Chromatogr. 1: 93-112. Stewart, G. , and Gierke, T. D.
The OH groups attached to the silicon atoms are reactive and mainly account for the adsorptive properties of silica gel. There are several different types of active sites, each varying in strength of adsorption. Substances are adsorbed on silica via hydrogen bonding and induced dipoledipole interactions, and the surface hydroxyls serve as hydrogen donors (Snyder, 1975). The surface pH of silica ranges from mildly acidic to neutral, ~ 5-7. According to Felton (1979), the most nearly "ideal" condition for general TLC silica gel separations is considered to be at the level of 11-12% water (by weight).
And Jackson, P. E. (1994). Principles and Practice of Modern Chromatographic Methods. Academic Press, San Diego, CA, pp. 179-226. Scott, R. P. , and Kucera, P. (1979). Solute-solvent interactions on the surface of silica gel. II. J. Chromatogr. 171: 37-48. Copyright © 1999 by Taylor & Francis Group, LLC Seidler, E. (1995). Experimental estimation of hydrophobic parameters of tetrazolium salts. Acta Histochem. 97: 295-299. , and Guiochon, G. (1981). Optimization in TLC—some practical considerations.
Thin-Layer Chromatography, Revised And Expanded (Chromatographic Science) by Bernard Fried