Joseph R. Lakowicz's Topics in Fluorescence Spectroscopy: Techniques PDF

By Joseph R. Lakowicz

ISBN-10: 0306438747

ISBN-13: 9780306438745

ISBN-10: 0306470578

ISBN-13: 9780306470578

During this inaugural quantity of a brand new sequence, specialists within the box aid biochemists, analytical chemists, spectroscopists, biophysicists, and different experts stay alongside of the newest recommendations and applied sciences to be had in fluorescence spectroscopy.

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Additional resources for Topics in Fluorescence Spectroscopy: Techniques

Sample text

The signal for the “start” discriminator can be derived by a number of methods. The minimum time jitter is usually obtained by viewing a fraction of the excitation pulse with a side-window photomultiplier or fast photodiode. In the case of synchronously pumped lasers, the electrical signal from the cavity dumper can also be used. The timing jitter on the “start” synchronization signal is much less than that of the “stop” photomultiplier due to the greater spread in pulse height from the latter.

The origin of the peak at 10 ns is still not clear though electron(79) and optical feedback (91) to the photocathode have been suggested as the cause. 12. In practice, we find that reconvolution is not affected by this peak provided its intensity is less than 2 % of that of the main peak. As regards the useful spectral range, we have performed single-photon timing experiments with the XP2020Q and its variants at fluorescence wavelengths from 230 up to 930 nm. (68) In comparison to linear focused photomultiplier tubes, side-window constructions have only been applied to fluorescence lifetime studies relatively recently.

This is clearly not insignificant, particularly when used with mode-locked laser pulses of a few picosecond duration and microchannel plate photomultipliers capable of having an instrumental response of The difference in pulse broadening across the grating between the excitation and fluorescence wavelengths can thus be an important source of error in reconvolution when measuring decay times of Unfortunately, it is at present inconvenient to tune the wavelength over a wide range in modelocked laser systems in order to overcome the problem by measuring the instrumental profile at the fluorescence wavelength.

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Topics in Fluorescence Spectroscopy: Techniques by Joseph R. Lakowicz


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